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KMID : 0545120100200030513
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 3 p.513 ~ p.517
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Immobilization on chitosan of a thermophilic trehalose synthase from Thermus thermophilus HJ6
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Kim Hyun-Jung
Kim Ae-Ran
Jeon Seong-Soo
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Abstract
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A thermostable trehalose synthase (TtTSase) from Thermus thermophilus HJ6 was immobilized on chitosan activated with glutaraldehyde. The yield of immobilization was evaluated as 39.68%. The optimum pH of the immobilized enzyme was similar to that of the free enzyme. However, the optimal temperature ranges were shifted of about 4°C due to better thermal stability after immobilization. The half-life of heat inactivation for free and immobilized enzymes was 5.7 and 6.3 days at 70 ºC, respectively, thus showing a lager thermostability of the immobilized enzyme. When tested in batch reaction, the immobilized enzyme retained its relative activity of 53% after 30 reuses of reaction within 12 days, and still retained 82% of its initial activity even after 150 days at 4 ºC. A packed-bed bioreactor with immobilized enzyme showed a maximum yield of 56% trehalose from 100mM maltose in continuous recycling system (bed volume: 10 mL) under conditions of pH 7.0 and 70 ºC.
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KEYWORD
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chitosan, immobilization, trehalose synthase, Thermus thermophilus, thermostability, bioreactor
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